Detailed information of TA system TA087148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1053461..1054155
• Replicon: chromosome
• Accession: NZ_CP024232
• Organism: Escherichia coli O6:H16 strain 2014EL-1346-6

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CNZ63_RS05605
• Protein ID: WP_001263493.1
• Coordinates: 1053461..1053859 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CNZ63_RS05610
• Protein ID: WP_000554757.1
• Coordinates: 1053862..1054155 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNZ63_RS05575	1048461..1049705	-	1245	WP_000189541.1	esterase FrsA	-
-	1049121..1049201	-	81	NuclAT_12	-	-
-	1049121..1049201	-	81	NuclAT_12	-	-
-	1049121..1049201	-	81	NuclAT_12	-	-
-	1049121..1049201	-	81	NuclAT_12	-	-
CNZ63_RS05580	1049797..1050255	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CNZ63_RS05585	1050516..1051973	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CNZ63_RS05590	1052030..1052551	-	522	Protein_998	peptide chain release factor H	-
CNZ63_RS05595	1052550..1052753	-	204	Protein_999	RNA ligase RtcB family protein	-
CNZ63_RS05600	1052999..1053451	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CNZ63_RS05605	1053461..1053859	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CNZ63_RS05610	1053862..1054155	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CNZ63_RS05615	1054207..1055262	-	1056	WP_001226164.1	DNA polymerase IV	-
CNZ63_RS05620	1055333..1056256	-	924	WP_001232570.1	putative lateral flagellar export/assembly protein LafU	-
CNZ63_RS05625	1056259..1057122	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
CNZ63_RS05630	1057135..1057854	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
CNZ63_RS05635	1057874..1058341	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T87148 WP_001263493.1 NZ_CP024232:c1053859-1053461 [Escherichia coli O6:H16]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T87148 NZ_CP024232:c1053859-1053461 [Escherichia coli O6:H16]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT87148 WP_000554757.1 NZ_CP024232:c1054155-1053862 [Escherichia coli O6:H16]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT87148 NZ_CP024232:c1054155-1053862 [Escherichia coli O6:H16]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6