Detailed information of TA system TA087141

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63508..63772
• Replicon: plasmid unnamed2
• Accession: NZ_CP024230
• Organism: Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: A3411_RS25965
• Protein ID: WP_001303307.1
• Coordinates: 63508..63660 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	63710..63772 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3411_RS25940	58762..60931	+	2170	Protein_63	DotA/TraY family protein	-
A3411_RS25945	61002..61664	+	663	WP_000644795.1	plasmid IncI1-type surface exclusion protein ExcA	-
A3411_RS25950	61736..61945	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
A3411_RS27010	62348..62524	+	177	WP_001054898.1	hypothetical protein	-
A3411_RS25955	62589..62885	-	297	WP_001275298.1	hypothetical protein	-
A3411_RS25960	63185..63436	+	252	WP_001291965.1	hypothetical protein	-
A3411_RS25965	63508..63660	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	63710..63772	+	63	NuclAT_0	-	Antitoxin
-	63710..63772	+	63	NuclAT_0	-	Antitoxin
-	63710..63772	+	63	NuclAT_0	-	Antitoxin
-	63710..63772	+	63	NuclAT_0	-	Antitoxin
A3411_RS25970	63975..65066	+	1092	WP_001756226.1	hypothetical protein	-
-	65136..65194	+	59	NuclAT_1	-	-
-	65136..65194	+	59	NuclAT_1	-	-
-	65136..65194	+	59	NuclAT_1	-	-
-	65136..65194	+	59	NuclAT_1	-	-
A3411_RS25975	65374..66582	+	1209	WP_001295719.1	IncI1-type conjugal transfer protein TrbA	-
A3411_RS25980	66601..67671	+	1071	WP_000151590.1	IncI1-type conjugal transfer protein TrbB	-
A3411_RS27015	67664..68359	+	696	WP_198406012.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrS1	-	1..73915	73915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T87141 WP_001303307.1 NZ_CP024230:c63660-63508 [Escherichia coli O25:NM]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T87141 NZ_CP024230:c63660-63508 [Escherichia coli O25:NM]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT87141 NZ_CP024230:63710-63772 [Escherichia coli O25:NM]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin