Detailed information of TA system TA087136

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 8092..8346
• Replicon: plasmid unnamed1
• Accession: NZ_CP024229
• Organism: Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: A3411_RS25375
• Protein ID: WP_001362723.1
• Coordinates: 8140..8346 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	8092..8153 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3411_RS25330	3268..3730	+	463	Protein_5	thermonuclease family protein	-
A3411_RS25335	3776..3985	+	210	WP_001353639.1	transcriptional regulator	-
A3411_RS25340	4023..4613	+	591	WP_074480776.1	DUF2726 domain-containing protein	-
A3411_RS25350	4949..6520	-	1572	WP_000381395.1	IS66 family transposase	-
A3411_RS25355	6540..6887	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
A3411_RS25360	6887..7565	-	679	Protein_10	IS66 family insertion sequence hypothetical protein	-
A3411_RS25365	7674..7940	+	267	WP_099583064.1	hypothetical protein	-
-	8092..8153	-	62	NuclAT_0	-	Antitoxin
-	8092..8153	-	62	NuclAT_0	-	Antitoxin
-	8092..8153	-	62	NuclAT_0	-	Antitoxin
-	8092..8153	-	62	NuclAT_0	-	Antitoxin
A3411_RS25375	8140..8346	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
A3411_RS25380	8630..8887	+	258	WP_000083826.1	replication regulatory protein RepA	-
A3411_RS25390	9122..9196	+	75	WP_011387702.1	RepA leader peptide Tap	-
A3411_RS25395	9189..10046	+	858	WP_074480778.1	incFII family plasmid replication initiator RepA	-
A3411_RS27000	10747..10926	+	180	WP_074483066.1	hypothetical protein	-
A3411_RS25410	11077..11664	-	588	Protein_17	IS91 family transposase	-
A3411_RS25420	11923..13104	+	1182	WP_001467544.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cofA	1..38467	38467
	-	inside	IScluster/Tn	-	-	198..18094	17896

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T87136 WP_001362723.1 NZ_CP024229:8140-8346 [Escherichia coli O25:NM]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T87136 NZ_CP024229:8140-8346 [Escherichia coli O25:NM]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATAGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT87136 NZ_CP024229:c8153-8092 [Escherichia coli O25:NM]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin