Detailed information of TA system TA087128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1997699..1998393
• Replicon: chromosome
• Accession: NZ_CP024228
• Organism: Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: A3411_RS10695
• Protein ID: WP_001263493.1
• Coordinates: 1997699..1998097 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A3411_RS10700
• Protein ID: WP_000554757.1
• Coordinates: 1998100..1998393 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3411_RS10665	1992699..1993943	-	1245	WP_000189541.1	esterase FrsA	-
-	1993359..1993439	-	81	NuclAT_11	-	-
-	1993359..1993439	-	81	NuclAT_11	-	-
-	1993359..1993439	-	81	NuclAT_11	-	-
-	1993359..1993439	-	81	NuclAT_11	-	-
A3411_RS10670	1994035..1994493	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A3411_RS10675	1994754..1996211	+	1458	WP_001293002.1	cytosol nonspecific dipeptidase	-
A3411_RS10680	1996268..1996789	-	522	Protein_1950	peptide chain release factor H	-
A3411_RS10685	1996788..1996991	-	204	Protein_1951	RNA ligase RtcB family protein	-
A3411_RS10690	1997237..1997689	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
A3411_RS10695	1997699..1998097	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A3411_RS10700	1998100..1998393	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A3411_RS10705	1998445..1999500	-	1056	WP_001226164.1	DNA polymerase IV	-
A3411_RS10710	1999571..2000356	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
A3411_RS10715	2000328..2002040	+	1713	Protein_1957	flagellar biosynthesis protein FlhA	-
A3411_RS10720	2002264..2002761	-	498	WP_000006242.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T87128 WP_001263493.1 NZ_CP024228:c1998097-1997699 [Escherichia coli O25:NM]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T87128 NZ_CP024228:c1998097-1997699 [Escherichia coli O25:NM]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT87128 WP_000554757.1 NZ_CP024228:c1998393-1998100 [Escherichia coli O25:NM]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT87128 NZ_CP024228:c1998393-1998100 [Escherichia coli O25:NM]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6