Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 102348..102850 Replicon chromosome
Accession NZ_CP024228
Organism Escherichia coli O25:NM strain 2014EL-1343-2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A3411_RS00490 Protein ID WP_000767829.1
Coordinates 102596..102850 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A3411_RS00485 Protein ID WP_001259255.1
Coordinates 102348..102599 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3411_RS00455 97526..98593 - 1068 WP_000080145.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
A3411_RS00460 98593..99663 - 1071 WP_000108954.1 histidinol-phosphate transaminase -
A3411_RS00465 99666..100964 - 1299 WP_001735396.1 histidinol dehydrogenase -
A3411_RS00470 100970..101869 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
A3411_RS00480 102015..102065 - 51 WP_001364200.1 his operon leader peptide -
A3411_RS00485 102348..102599 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A3411_RS00490 102596..102850 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A3411_RS00495 102933..103757 + 825 WP_000754737.1 SDR family oxidoreductase -
A3411_RS00500 103803..104732 + 930 WP_000803337.1 LysR family transcriptional regulator -
A3411_RS00505 104947..105009 + 63 WP_010723108.1 membrane protein YoeI -
A3411_RS00510 104999..106357 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A3411_RS00515 106536..107594 + 1059 WP_000492340.1 transport protein YeeE -
A3411_RS00520 107608..107835 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T87116 WP_000767829.1 NZ_CP024228:102596-102850 [Escherichia coli O25:NM]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T87116 NZ_CP024228:102596-102850 [Escherichia coli O25:NM]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT87116 WP_001259255.1 NZ_CP024228:102348-102599 [Escherichia coli O25:NM]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT87116 NZ_CP024228:102348-102599 [Escherichia coli O25:NM]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References