Detailed information of TA system TA087114

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 104470..104724
• Replicon: plasmid unnamed4
• Accession: NZ_CP024227
• Organism: Escherichia coli O169:H41 strain 2014EL-1345-2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CJV63_RS26705
• Protein ID: WP_001362723.1
• Coordinates: 104470..104676 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	104668..104724 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJV63_RS26665	100865..101511	-	647	Protein_119	IS630 family transposase	-
CJV63_RS26670	101512..101913	+	402	Protein_120	IS91 family transposase	-
CJV63_RS26685	102776..103633	-	858	WP_001401515.1	incFII family plasmid replication initiator RepA	-
CJV63_RS26690	103626..103700	-	75	WP_001365705.1	RepA leader peptide Tap	-
CJV63_RS26700	103926..104186	-	261	WP_000083817.1	replication regulatory protein RepA	-
CJV63_RS26705	104470..104676	-	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	104668..104724	+	57	NuclAT_1	-	Antitoxin
-	104668..104724	+	57	NuclAT_1	-	Antitoxin
-	104668..104724	+	57	NuclAT_1	-	Antitoxin
-	104668..104724	+	57	NuclAT_1	-	Antitoxin
CJV63_RS26715	104968..105150	+	183	WP_001393357.1	hypothetical protein	-
CJV63_RS26720	105374..106105	-	732	WP_001717613.1	type-F conjugative transfer system pilin acetylase TraX	-
CJV63_RS26725	106102..106648	-	547	Protein_127	DUF2726 domain-containing protein	-
CJV63_RS26730	106666..109503	-	2838	Protein_128	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeE / faeD / cofA / cssA / estIa	1..145086	145086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T87114 WP_001362723.1 NZ_CP024227:c104676-104470 [Escherichia coli O169:H41]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T87114 NZ_CP024227:c104676-104470 [Escherichia coli O169:H41]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACCTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT87114 NZ_CP024227:104668-104724 [Escherichia coli O169:H41]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCCTTTCT

Structures

Antitoxin