Detailed information of TA system TA087109

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75476..75740
• Replicon: plasmid unnamed3
• Accession: NZ_CP024226
• Organism: Escherichia coli O169:H41 strain 2014EL-1345-2

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: CJV63_RS25990
• Protein ID: WP_001303307.1
• Coordinates: 75476..75628 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	75678..75740 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJV63_RS25965	70732..72900	+	2169	WP_000698356.1	DotA/TraY family protein	-
CJV63_RS25970	72971..73633	+	663	WP_000644795.1	plasmid IncI1-type surface exclusion protein ExcA	-
CJV63_RS25975	73705..73914	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CJV63_RS27490	74316..74492	+	177	WP_001054898.1	hypothetical protein	-
CJV63_RS25980	74557..74853	-	297	WP_001275298.1	hypothetical protein	-
CJV63_RS25985	75153..75404	+	252	WP_001291965.1	hypothetical protein	-
CJV63_RS25990	75476..75628	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	75678..75740	+	63	NuclAT_0	-	Antitoxin
-	75678..75740	+	63	NuclAT_0	-	Antitoxin
-	75678..75740	+	63	NuclAT_0	-	Antitoxin
-	75678..75740	+	63	NuclAT_0	-	Antitoxin
CJV63_RS25995	75943..77034	+	1092	WP_001756226.1	hypothetical protein	-
-	77104..77162	+	59	NuclAT_1	-	-
-	77104..77162	+	59	NuclAT_1	-	-
-	77104..77162	+	59	NuclAT_1	-	-
-	77104..77162	+	59	NuclAT_1	-	-
CJV63_RS26000	77342..78550	+	1209	WP_001295719.1	IncI1-type conjugal transfer protein TrbA	-
CJV63_RS26005	78569..79639	+	1071	WP_000151590.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / qnrS1	-	1..85864	85864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T87109 WP_001303307.1 NZ_CP024226:c75628-75476 [Escherichia coli O169:H41]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T87109 NZ_CP024226:c75628-75476 [Escherichia coli O169:H41]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT87109 NZ_CP024226:75678-75740 [Escherichia coli O169:H41]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin