Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3334062..3334603 Replicon chromosome
Accession NZ_CP024223
Organism Escherichia coli O169:H41 strain 2014EL-1345-2

Toxin (Protein)


Gene name yafQ Uniprot ID A0A3W4DAB6
Locus tag CJV63_RS16750 Protein ID WP_024171431.1
Coordinates 3334062..3334340 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CJV63_RS16755 Protein ID WP_000729704.1
Coordinates 3334343..3334603 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJV63_RS16735 3331644..3332222 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CJV63_RS16740 3332428..3333195 + 768 WP_000333380.1 class II glutamine amidotransferase -
CJV63_RS16745 3333166..3333906 - 741 WP_001225679.1 murein L,D-transpeptidase -
CJV63_RS16750 3334062..3334340 - 279 WP_024171431.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CJV63_RS16755 3334343..3334603 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CJV63_RS16760 3334813..3335562 + 750 WP_000056850.1 C40 family peptidase -
CJV63_RS16765 3335738..3336235 + 498 WP_001612413.1 REP-associated tyrosine transposase RayT -
CJV63_RS16770 3336422..3338134 - 1713 Protein_3076 flagellar biosynthesis protein FlhA -
CJV63_RS16775 3338106..3338891 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - tssA / gmhA/lpcA 3315865..3336235 20370


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10829.68 Da        Isoelectric Point: 10.3349

>T87103 WP_024171431.1 NZ_CP024223:c3334340-3334062 [Escherichia coli O169:H41]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVKPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T87103 NZ_CP024223:c3334340-3334062 [Escherichia coli O169:H41]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCAAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT87103 WP_000729704.1 NZ_CP024223:c3334603-3334343 [Escherichia coli O169:H41]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT87103 NZ_CP024223:c3334603-3334343 [Escherichia coli O169:H41]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W4DAB6

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References