Detailed information of TA system TA087042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2599034..2599647
• Replicon: chromosome
• Accession: NZ_CP024173
• Organism: Bordetella bronchiseptica strain D987

Toxin (Protein)

• Gene name: higB
• Uniprot ID: K0MEM3
• Locus tag: CS345_RS12245
• Protein ID: WP_003811545.1
• Coordinates: 2599034..2599345 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CS345_RS12250
• Protein ID: WP_003811547.1
• Coordinates: 2599348..2599647 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS345_RS12220	2594175..2594741	-	567	WP_015039900.1	LamB/YcsF family protein	-
CS345_RS12225	2594738..2596132	-	1395	WP_010926625.1	arylsulfatase	-
CS345_RS12230	2596147..2597109	-	963	WP_003811538.1	tripartite tricarboxylate transporter substrate binding protein	-
CS345_RS12235	2597225..2598178	+	954	WP_015063803.1	LysR family transcriptional regulator	-
CS345_RS12240	2598297..2598920	+	624	WP_003811542.1	glutathione S-transferase family protein	-
CS345_RS12245	2599034..2599345	+	312	WP_003811545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CS345_RS12250	2599348..2599647	+	300	WP_003811547.1	helix-turn-helix domain-containing protein	Antitoxin
CS345_RS12255	2599661..2602732	-	3072	Protein_2414	autotransporter SphB2	-
CS345_RS12260	2602862..2603536	+	675	WP_015063801.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-96)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11810.72 Da
• Isoelectric Point: 8.8245

>T87042 WP_003811545.1 NZ_CP024173:2599034-2599345 [Bordetella bronchiseptica]
MEFIETPIFTQLITALLSDEEYSRLQQLLIDNPERGDLLRGGGGIRKMRYGRQGTGKRGGIRVIYYWISQDCQIYMLAAY
AKSKKINLTPDEIAALRELVKEL

Nucleotide

• Length: 312 bp

>T87042 NZ_CP024173:2599034-2599345 [Bordetella bronchiseptica]
ATGGAGTTCATCGAAACCCCAATTTTCACGCAATTGATCACCGCGTTGCTGTCGGATGAGGAATATTCCAGGCTGCAACA
GCTGCTGATCGACAACCCCGAAAGGGGCGATCTGCTGCGTGGGGGCGGCGGTATACGAAAGATGCGTTACGGCCGGCAAG
GCACGGGCAAGCGAGGTGGCATCCGGGTCATTTATTACTGGATCAGCCAGGATTGTCAGATCTACATGCTTGCGGCTTAT
GCAAAGTCGAAGAAGATCAACCTGACCCCGGACGAAATCGCGGCGCTGCGCGAACTGGTCAAGGAGCTGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10946.70 Da
• Isoelectric Point: 11.2467

>AT87042 WP_003811547.1 NZ_CP024173:2599348-2599647 [Bordetella bronchiseptica]
MKKDNTVFNDLQASLKQAVAIRQGKMPPSRVFKVETVDVKATREKTHMSQTEFAKAIHVSVRTLQNWEQGHRNPTGPAVA
LLKIVASAPDIAVRALRTA

Nucleotide

• Length: 300 bp

>AT87042 NZ_CP024173:2599348-2599647 [Bordetella bronchiseptica]
ATGAAAAAAGACAACACGGTGTTCAACGACCTGCAAGCCAGCCTGAAGCAAGCCGTGGCCATTCGCCAAGGCAAGATGCC
GCCATCGCGTGTATTCAAGGTTGAGACTGTGGATGTGAAGGCAACGCGCGAAAAAACGCACATGTCTCAAACGGAATTCG
CCAAAGCCATCCACGTCAGTGTCCGTACGCTGCAGAACTGGGAGCAAGGTCACCGCAATCCGACGGGCCCTGCCGTGGCG
CTTCTGAAAATCGTGGCAAGCGCCCCGGACATTGCCGTGCGAGCGCTGCGGACCGCGTAA

Structures

Toxin

• AlphaFold DB: K0MEM3