Detailed information of TA system TA086979

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28013..28277
• Replicon: plasmid p14EC033e
• Accession: NZ_CP024152
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: CR539_RS26205
• Protein ID: WP_001331364.1
• Coordinates: 28013..28165 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	28220..28277 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS26185	23347..25515	+	2169	WP_000698366.1	DotA/TraY family protein	-
CR539_RS26190	25580..26242	+	663	WP_000644792.1	plasmid IncI1-type surface exclusion protein ExcA	-
CR539_RS26195	26314..26523	-	210	WP_000062602.1	HEAT repeat domain-containing protein	-
CR539_RS28325	26855..27031	+	177	WP_001054900.1	hypothetical protein	-
CR539_RS26200	27690..27941	+	252	WP_001291964.1	hypothetical protein	-
CR539_RS26205	28013..28165	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	28220..28277	+	58	NuclAT_0	-	Antitoxin
-	28220..28277	+	58	NuclAT_0	-	Antitoxin
-	28220..28277	+	58	NuclAT_0	-	Antitoxin
-	28220..28277	+	58	NuclAT_0	-	Antitoxin
CR539_RS26210	28438..29424	+	987	WP_001257838.1	hypothetical protein	-
CR539_RS26215	29642..30850	+	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
CR539_RS26220	30869..31939	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..87351	87351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T86979 WP_001331364.1 NZ_CP024152:c28165-28013 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T86979 NZ_CP024152:c28165-28013 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT86979 NZ_CP024152:28220-28277 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin