Detailed information of TA system TA086975

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 78025..78451
• Replicon: plasmid p14EC033d
• Accession: NZ_CP024151
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CR539_RS25880
• Protein ID: WP_001312861.1
• Coordinates: 78025..78183 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	78227..78451 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS25835	73073..73300	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
CR539_RS25840	73395..74081	-	687	WP_015059009.1	PAS domain-containing protein	-
CR539_RS25845	74272..74655	-	384	WP_000124981.1	relaxosome protein TraM	-
CR539_RS25850	74932..75579	+	648	WP_148726238.1	transglycosylase SLT domain-containing protein	-
CR539_RS25855	75876..76697	-	822	WP_033804334.1	DUF945 domain-containing protein	-
CR539_RS25860	76816..77103	-	288	WP_000107535.1	hypothetical protein	-
CR539_RS28315	77128..77334	-	207	WP_033804333.1	hypothetical protein	-
CR539_RS25880	78025..78183	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	78227..78451	-	225	NuclAT_0	-	Antitoxin
-	78227..78451	-	225	NuclAT_0	-	Antitoxin
-	78227..78451	-	225	NuclAT_0	-	Antitoxin
-	78227..78451	-	225	NuclAT_0	-	Antitoxin
CR539_RS28130	78263..78451	+	189	WP_063073474.1	hypothetical protein	-
CR539_RS25885	78463..79182	-	720	WP_001276240.1	plasmid SOS inhibition protein A	-
CR539_RS25890	79179..79613	-	435	WP_000845907.1	conjugation system SOS inhibitor PsiB	-
CR539_RS25895	79668..81626	-	1959	WP_063072993.1	ParB/RepB/Spo0J family partition protein	-
CR539_RS25900	81691..81924	-	234	WP_000006030.1	DUF905 family protein	-
CR539_RS25905	81986..82438	-	453	WP_000290842.1	single-stranded DNA-binding protein	-
CR539_RS28320	82464..82670	-	207	WP_000275853.1	hypothetical protein	-
CR539_RS25930	83081..83305	-	225	WP_016238251.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..97858	97858

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86975 WP_001312861.1 NZ_CP024151:c78183-78025 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86975 NZ_CP024151:c78183-78025 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT86975 NZ_CP024151:c78451-78227 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGACAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin