Detailed information of TA system TA086970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 49218..49886
• Replicon: plasmid p14EC033c
• Accession: NZ_CP024150
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3Q0N4Z5
• Locus tag: CR539_RS25040
• Protein ID: WP_044789354.1
• Coordinates: 49218..49541 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CR539_RS25045
• Protein ID: WP_044789356.1
• Coordinates: 49590..49886 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS25020	45835..46362	+	528	WP_044789347.1	superoxide dismutase family protein	-
CR539_RS25030	46688..48436	-	1749	WP_044789349.1	NERD domain-containing protein	-
CR539_RS25035	48591..48965	-	375	Protein_52	hypothetical protein	-
CR539_RS25040	49218..49541	+	324	WP_044789354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CR539_RS25045	49590..49886	+	297	WP_044789356.1	helix-turn-helix domain-containing protein	Antitoxin
CR539_RS25050	50327..50959	-	633	WP_044789357.1	recombinase family protein	-
CR539_RS25055	51381..51905	+	525	WP_044789358.1	hypothetical protein	-
CR539_RS25060	52178..52534	+	357	WP_044789359.1	hypothetical protein	-
CR539_RS25065	52614..53433	+	820	Protein_58	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108710	108710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (46-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12358.31 Da
• Isoelectric Point: 9.6090

>T86970 WP_044789354.1 NZ_CP024150:49218-49541 [Escherichia coli]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCKKIRWSRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDDLSEKEKAVMKVLAQQLK

Nucleotide

• Length: 324 bp

>T86970 NZ_CP024150:49218-49541 [Escherichia coli]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGTCTGCGTAAAGAGCTCATGGATGATGATGAATTTCGGGA
ACTGCAGACCTATTTACTGGAAGCTCATGATCGGGGTGACACTATCAGTCACACAGGTGGATGCAAAAAGATTCGGTGGA
GCCGCCCAGGGATGGGGAAGCGAGGTGGCGTCAGAGTGATTTACTACGTTCGCCTTGCCAGCGGCAGATTATACCTGCTT
CTGATATACCCTAAGAATGCTAAAGATGATCTTAGCGAGAAAGAAAAAGCCGTTATGAAGGTATTAGCACAGCAGCTGAA
ATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11118.03 Da
• Isoelectric Point: 7.2804

>AT86970 WP_044789356.1 NZ_CP024150:49590-49886 [Escherichia coli]
MEKKLFDALINSMEEMVAIENGQIQPAEENIHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLIRSWEQKRRIPSGIALK
MLCLIEREPALINTLRSI

Nucleotide

• Length: 297 bp

>AT86970 NZ_CP024150:49590-49886 [Escherichia coli]
ATGGAAAAGAAACTTTTTGATGCACTTATTAACAGCATGGAGGAAATGGTTGCCATTGAGAATGGCCAAATCCAGCCAGC
TGAAGAAAATATTCACCGCCATACACTGCCGGATGTGAAGGCTATTCGTAAACATGCCGGAATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGAGCCAGCGTTGACCTTATCCGCAGCTGGGAACAGAAACGGCGTATCCCGTCGGGTATTGCGTTGAAA
ATGCTCTGTCTCATCGAACGTGAGCCAGCACTGATTAATACCTTGCGCAGCATTTAA

Structures

Toxin

• AlphaFold DB: A0A3Q0N4Z5