Detailed information of TA system TA086965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4057977..4058776
• Replicon: chromosome
• Accession: NZ_CP024147
• Organism: Escherichia coli strain 14EC033

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: CR539_RS21005
• Protein ID: WP_000347273.1
• Coordinates: 4058312..4058776 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CR539_RS21000
• Protein ID: WP_001307405.1
• Coordinates: 4057977..4058312 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR539_RS20985	4053762..4054532	-	771	WP_024250085.1	2-dehydro-3-deoxyglucarate aldolase	-
CR539_RS20990	4054548..4055882	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
CR539_RS20995	4056257..4057828	+	1572	WP_032281643.1	galactarate dehydratase	-
CR539_RS21000	4057977..4058312	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CR539_RS21005	4058312..4058776	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CR539_RS21010	4058831..4059640	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CR539_RS21020	4059889..4061169	+	1281	WP_000681944.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CR539_RS21025	4061192..4061665	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CR539_RS21030	4061676..4062455	+	780	WP_101969530.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CR539_RS21035	4062445..4063323	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CR539_RS21040	4063341..4063775	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4049059..4058776	9717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T86965 WP_000347273.1 NZ_CP024147:4058312-4058776 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T86965 NZ_CP024147:4058312-4058776 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT86965 WP_001307405.1 NZ_CP024147:4057977-4058312 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT86965 NZ_CP024147:4057977-4058312 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCTTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: A0A0E0XVC7