Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2310641..2311203 Replicon chromosome
Accession NZ_CP024147
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag CR539_RS11990 Protein ID WP_000605676.1
Coordinates 2310925..2311203 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag CR539_RS11985 Protein ID WP_000781370.1
Coordinates 2310641..2310925 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS11970 2305823..2308870 + 3048 WP_012767733.1 formate dehydrogenase-N subunit alpha -
CR539_RS11975 2308883..2309767 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
CR539_RS11980 2309760..2310413 + 654 WP_032280861.1 formate dehydrogenase-N subunit gamma -
CR539_RS11985 2310641..2310925 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
CR539_RS11990 2310925..2311203 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR539_RS11995 2311389..2312399 - 1011 WP_101969391.1 alcohol dehydrogenase AdhP -
CR539_RS12000 2312533..2314230 - 1698 WP_000433464.1 malate dehydrogenase -
CR539_RS12005 2314386..2314523 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
CR539_RS12010 2314625..2314840 - 216 WP_101969392.1 biofilm-dependent modulation protein -
CR539_RS12020 2315186..2315617 + 432 WP_032280863.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T86952 WP_000605676.1 NZ_CP024147:c2311203-2310925 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T86952 NZ_CP024147:c2311203-2310925 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT86952 WP_000781370.1 NZ_CP024147:c2310925-2310641 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT86952 NZ_CP024147:c2310925-2310641 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References