Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1048972..1049666 Replicon chromosome
Accession NZ_CP024147
Organism Escherichia coli strain 14EC033

Toxin (Protein)


Gene name yafO Uniprot ID A0A080JBV5
Locus tag CR539_RS05365 Protein ID WP_032280310.1
Coordinates 1049268..1049666 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CR539_RS05360 Protein ID WP_000554758.1
Coordinates 1048972..1049265 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR539_RS05340 1044696..1045193 + 498 WP_032280308.1 transposase -
CR539_RS05345 1045325..1047037 - 1713 Protein_952 flagellar biosynthesis protein FlhA -
CR539_RS05350 1047009..1047794 + 786 WP_162721673.1 putative lateral flagellar export/assembly protein LafU -
CR539_RS05355 1047865..1048920 + 1056 WP_101969331.1 DNA polymerase IV -
CR539_RS05360 1048972..1049265 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CR539_RS05365 1049268..1049666 + 399 WP_032280310.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CR539_RS05370 1049676..1049933 + 258 Protein_957 GNAT family N-acetyltransferase -
CR539_RS05375 1049989..1050572 + 584 Protein_958 IS1 family transposase -
CR539_RS05385 1050605..1051818 + 1214 WP_089602041.1 IS3 family transposase -
CR539_RS28215 1051825..1051947 + 123 Protein_960 IS1 family transposase -
CR539_RS28220 1051963..1052166 + 204 Protein_961 GNAT family N-acetyltransferase -
CR539_RS05395 1052484..1052692 + 209 Protein_962 RtcB family protein -
CR539_RS05400 1052712..1053020 + 309 Protein_963 peptide chain release factor H -
CR539_RS05405 1053077..1054534 - 1458 WP_001293010.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1047865..1080764 32899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15501.91 Da        Isoelectric Point: 8.0949

>T86947 WP_032280310.1 NZ_CP024147:1049268-1049666 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTVDFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T86947 NZ_CP024147:1049268-1049666 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGTGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT86947 WP_000554758.1 NZ_CP024147:1048972-1049265 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT86947 NZ_CP024147:1048972-1049265 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A080JBV5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References