Detailed information of TA system TA086937

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27401..27822
• Replicon: plasmid p14EC029d
• Accession: NZ_CP024145
• Organism: Escherichia coli strain 14EC029

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CR538_RS28550
• Protein ID: WP_001302184.1
• Coordinates: 27664..27822 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	27401..27599 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR538_RS28510	22615..23041	-	427	Protein_28	hypothetical protein	-
CR538_RS30135	23111..23317	+	207	WP_000275848.1	hypothetical protein	-
CR538_RS28525	23343..23882	+	540	WP_000290811.1	single-stranded DNA-binding protein	-
CR538_RS28530	23959..24162	+	204	WP_101968913.1	DUF905 family protein	-
CR538_RS28535	24226..26184	+	1959	WP_000117204.1	ParB/RepB/Spo0J family partition protein	-
CR538_RS28540	26239..26673	+	435	WP_000845912.1	conjugation system SOS inhibitor PsiB	-
CR538_RS28545	26670..27389	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	27401..27599	+	199	NuclAT_0	-	Antitoxin
-	27401..27599	+	199	NuclAT_0	-	Antitoxin
-	27401..27599	+	199	NuclAT_0	-	Antitoxin
-	27401..27599	+	199	NuclAT_0	-	Antitoxin
CR538_RS28550	27664..27822	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
CR538_RS30140	28123..28309	-	187	Protein_36	pilus protein	-
CR538_RS28560	28369..28656	+	288	WP_000107541.1	hypothetical protein	-
CR538_RS28565	28774..29595	+	822	WP_001424949.1	DUF945 domain-containing protein	-
CR538_RS28570	29891..30493	-	603	WP_077632063.1	transglycosylase SLT domain-containing protein	-
CR538_RS28575	30815..31198	+	384	WP_001151534.1	relaxosome protein TraM	-
CR538_RS28580	31389..32036	+	648	WP_000332521.1	conjugal transfer transcriptional regulator TraJ	-
CR538_RS28585	32172..32387	+	216	WP_071782156.1	conjugal transfer relaxosome protein TraY	-
CR538_RS28590	32431..32796	+	366	WP_000338819.1	type IV conjugative transfer system pilin TraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltB / eltA	1..106478	106478

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T86937 WP_001302184.1 NZ_CP024145:27664-27822 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86937 NZ_CP024145:27664-27822 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT86937 NZ_CP024145:27401-27599 [Escherichia coli]
TCATACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin