Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1899030..1899532 Replicon chromosome
Accession NZ_CP024141
Organism Escherichia coli strain 14EC029

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CR538_RS09740 Protein ID WP_000767829.1
Coordinates 1899278..1899532 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CR538_RS09735 Protein ID WP_001259255.1
Coordinates 1899030..1899281 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR538_RS09705 1894208..1895275 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CR538_RS09710 1895275..1896345 - 1071 WP_001362957.1 histidinol-phosphate transaminase -
CR538_RS09715 1896342..1897646 - 1305 WP_001774536.1 histidinol dehydrogenase -
CR538_RS09720 1897652..1898551 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CR538_RS09730 1898697..1898747 - 51 WP_001364200.1 his operon leader peptide -
CR538_RS09735 1899030..1899281 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CR538_RS09740 1899278..1899532 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CR538_RS09745 1899615..1900439 + 825 WP_000754758.1 SDR family oxidoreductase -
CR538_RS09750 1900485..1901414 + 930 WP_101968763.1 LysR family transcriptional regulator -
CR538_RS09755 1901629..1901691 + 63 WP_010723108.1 membrane protein YoeI -
CR538_RS09760 1901681..1903039 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CR538_RS09765 1903218..1904276 + 1059 WP_000492315.1 transport protein YeeE -
CR538_RS09770 1904290..1904517 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86918 WP_000767829.1 NZ_CP024141:1899278-1899532 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86918 NZ_CP024141:1899278-1899532 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86918 WP_001259255.1 NZ_CP024141:1899030-1899281 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86918 NZ_CP024141:1899030-1899281 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References