Detailed information of TA system TA086907

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 123151..123577
• Replicon: plasmid p14EC020b
• Accession: NZ_CP024140
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CR537_RS26655
• Protein ID: WP_001312861.1
• Coordinates: 123151..123309 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	123353..123577 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS26620	118525..119214	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
CR537_RS26625	119401..119784	-	384	WP_001151524.1	relaxosome protein TraM	-
CR537_RS26630	120105..120707	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
CR537_RS26635	121004..121825	-	822	WP_001234426.1	DUF945 domain-containing protein	-
CR537_RS26640	121943..122230	-	288	WP_000107535.1	hypothetical protein	-
CR537_RS26655	123151..123309	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	123353..123577	-	225	NuclAT_0	-	Antitoxin
-	123353..123577	-	225	NuclAT_0	-	Antitoxin
-	123353..123577	-	225	NuclAT_0	-	Antitoxin
-	123353..123577	-	225	NuclAT_0	-	Antitoxin
CR537_RS27225	123389..123577	+	189	WP_001299721.1	hypothetical protein	-
CR537_RS26660	123589..124308	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
CR537_RS26665	124305..124739	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
CR537_RS26670	124794..124991	-	198	Protein_144	hypothetical protein	-
CR537_RS26675	125019..125252	-	234	WP_000005987.1	DUF905 family protein	-
CR537_RS26680	125320..125859	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
CR537_RS27375	125885..126091	-	207	WP_000275856.1	hypothetical protein	-
CR537_RS26705	127230..128201	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / sul3 / dfrA14 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / aac(3)-IId / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..166233	166233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86907 WP_001312861.1 NZ_CP024140:c123309-123151 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86907 NZ_CP024140:c123309-123151 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT86907 NZ_CP024140:c123577-123353 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin