Detailed information of TA system TA086902

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83561..83815
• Replicon: plasmid p14EC020b
• Accession: NZ_CP024140
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CR537_RS26415
• Protein ID: WP_001351576.1
• Coordinates: 83561..83767 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83754..83815 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS26375	79888..80235	-	348	WP_000612556.1	IS66 family insertion sequence element accessory protein TnpB	-
CR537_RS26380	80232..80612	-	381	WP_001171523.1	IS66 family insertion sequence hypothetical protein	-
CR537_RS26390	81389..82246	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
CR537_RS26395	82239..82721	-	483	WP_181726436.1	hypothetical protein	-
CR537_RS26400	82714..82788	-	75	WP_001442103.1	RepA leader peptide Tap	-
CR537_RS26410	83020..83277	-	258	WP_000083845.1	replication regulatory protein RepA	-
CR537_RS26415	83561..83767	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	83754..83815	+	62	NuclAT_1	-	Antitoxin
-	83754..83815	+	62	NuclAT_1	-	Antitoxin
-	83754..83815	+	62	NuclAT_1	-	Antitoxin
-	83754..83815	+	62	NuclAT_1	-	Antitoxin
CR537_RS27365	84071..84145	-	75	Protein_96	endonuclease	-
CR537_RS26425	84391..84603	-	213	WP_001299730.1	ANR family transcriptional regulator	-
CR537_RS26430	84739..85299	-	561	WP_000139315.1	fertility inhibition protein FinO	-
CR537_RS26435	85402..86262	-	861	WP_000704514.1	alpha/beta hydrolase	-
CR537_RS26440	86321..87067	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / sul3 / dfrA14 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / aac(3)-IId / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..166233	166233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T86902 WP_001351576.1 NZ_CP024140:c83767-83561 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T86902 NZ_CP024140:c83767-83561 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT86902 NZ_CP024140:83754-83815 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin