Detailed information of TA system TA086897

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4695005..4695607
• Replicon: chromosome
• Accession: NZ_CP024138
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CR537_RS24380
• Protein ID: WP_000897305.1
• Coordinates: 4695296..4695607 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CR537_RS24375
• Protein ID: WP_000356397.1
• Coordinates: 4695005..4695295 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS24355	4691507..4692409	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CR537_RS24360	4692406..4693041	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CR537_RS24365	4693038..4693967	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CR537_RS24370	4694182..4694400	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CR537_RS24375	4695005..4695295	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CR537_RS24380	4695296..4695607	-	312	WP_000897305.1	hypothetical protein	Toxin
CR537_RS24385	4695836..4696744	+	909	WP_001318161.1	alpha/beta hydrolase	-
CR537_RS24390	4696808..4697749	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CR537_RS24395	4697794..4698231	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CR537_RS24400	4698228..4699100	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CR537_RS24405	4699094..4699693	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T86897 WP_000897305.1 NZ_CP024138:c4695607-4695296 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T86897 NZ_CP024138:c4695607-4695296 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT86897 WP_000356397.1 NZ_CP024138:c4695295-4695005 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT86897 NZ_CP024138:c4695295-4695005 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7