Detailed information of TA system TA086885

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2436746..2436971
• Replicon: chromosome
• Accession: NZ_CP024138
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CR537_RS12565
• Protein ID: WP_000813254.1
• Coordinates: 2436816..2436971 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2436746..2436804 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS12530	2431914..2432321	-	408	WP_000381212.1	helix-turn-helix domain-containing protein	-
CR537_RS12535	2432402..2432629	+	228	WP_000921596.1	transcriptional regulator	-
CR537_RS12540	2432613..2433134	+	522	WP_000705360.1	hypothetical protein	-
CR537_RS12545	2433115..2434080	+	966	WP_000054495.1	hypothetical protein	-
CR537_RS12550	2434121..2434522	+	402	WP_001151252.1	DUF977 family protein	-
CR537_RS12555	2435056..2436081	+	1026	WP_000547812.1	RelA/SpoT domain-containing protein	-
-	2436746..2436804	-	59	-	-	Antitoxin
CR537_RS12565	2436816..2436971	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
CR537_RS12570	2437188..2437439	+	252	WP_000980999.1	hypothetical protein	-
CR537_RS12575	2437506..2437784	+	279	WP_032297225.1	hypothetical protein	-
CR537_RS12580	2437786..2438835	+	1050	WP_001696469.1	DUF968 domain-containing protein	-
CR537_RS12585	2438849..2439601	+	753	WP_001047132.1	antitermination protein	-
CR537_RS12590	2439776..2439978	-	203	Protein_2302	cold shock-like protein CspF	-
CR537_RS12595	2440277..2440492	+	216	WP_000066485.1	cold shock-like protein CspB	-
CR537_RS27440	2440856..2441026	-	171	WP_001405948.1	putative zinc-binding protein YnfU	-
CR537_RS12605	2441246..2441461	+	216	WP_000839590.1	phage lysis protein EssD	-
CR537_RS12610	2441466..2441777	+	312	WP_000189916.1	YdfR family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2421928..2478082	56154

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T86885 WP_000813254.1 NZ_CP024138:2436816-2436971 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T86885 NZ_CP024138:2436816-2436971 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT86885 NZ_CP024138:c2436804-2436746 [Escherichia coli]
CCTTGCCTTCCGGCACGTAAGAGGCTAACCTACATGTGTCTAGCATGAAATTGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin