Detailed information of TA system TA086875

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2015710..2015935
• Replicon: chromosome
• Accession: NZ_CP024138
• Organism: Escherichia coli strain 14EC020

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CR537_RS10170
• Protein ID: WP_000813254.1
• Coordinates: 2015780..2015935 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2015710..2015768 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR537_RS10125	2011088..2011306	-	219	WP_001171942.1	hypothetical protein	-
CR537_RS10135	2011466..2011621	-	156	WP_000379575.1	DUF1391 family protein	-
CR537_RS10140	2011919..2012338	-	420	WP_000233320.1	helix-turn-helix domain-containing protein	-
CR537_RS10145	2012418..2012672	+	255	WP_001072337.1	hypothetical protein	-
CR537_RS10150	2012669..2013094	+	426	WP_000693853.1	Rha family transcriptional regulator	-
CR537_RS10155	2013166..2014236	+	1071	WP_021530548.1	hypothetical protein	-
CR537_RS10160	2014277..2014702	+	426	WP_001151253.1	DUF977 family protein	-
CR537_RS10165	2014877..2015542	+	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
-	2015710..2015768	-	59	-	-	Antitoxin
CR537_RS10170	2015780..2015935	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
CR537_RS10175	2016103..2016381	+	279	WP_024193993.1	hypothetical protein	-
CR537_RS10180	2016383..2017441	+	1059	WP_101980497.1	DUF968 domain-containing protein	-
CR537_RS10185	2017442..2017807	+	366	WP_000140004.1	RusA family crossover junction endodeoxyribonuclease	-
CR537_RS10190	2017804..2018493	+	690	WP_101980498.1	antiterminator	-
CR537_RS10220	2019306..2019521	+	216	WP_000839572.1	class II holin family protein	-
CR537_RS10225	2019526..2019876	+	351	WP_059333431.1	YdfR family protein	-
CR537_RS10230	2019940..2020473	+	534	WP_021568386.1	lysozyme	-
CR537_RS10240	2020690..2020872	+	183	WP_077575695.1	Rz1 family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ybtP / ybtQ / ybtX / ybtS	1997269..2047677	50408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T86875 WP_000813254.1 NZ_CP024138:2015780-2015935 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T86875 NZ_CP024138:2015780-2015935 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT86875 NZ_CP024138:c2015768-2015710 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin