Detailed information of TA system TA086853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4073158..4073852
• Replicon: chromosome
• Accession: NZ_CP024134
• Organism: Escherichia coli strain 14EC017

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CR536_RS22010
• Protein ID: WP_001263493.1
• Coordinates: 4073158..4073556 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CR536_RS22015
• Protein ID: WP_000554757.1
• Coordinates: 4073559..4073852 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR536_RS21980	4068158..4069402	-	1245	WP_000189541.1	esterase FrsA	-
-	4068818..4068898	-	81	NuclAT_9	-	-
-	4068818..4068898	-	81	NuclAT_9	-	-
-	4068818..4068898	-	81	NuclAT_9	-	-
-	4068818..4068898	-	81	NuclAT_9	-	-
CR536_RS21985	4069494..4069952	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CR536_RS21990	4070213..4071670	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CR536_RS21995	4071727..4072248	-	522	Protein_3999	peptide chain release factor H	-
CR536_RS22000	4072247..4072450	-	204	Protein_4000	RNA ligase RtcB family protein	-
CR536_RS22005	4072696..4073148	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CR536_RS22010	4073158..4073556	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CR536_RS22015	4073559..4073852	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CR536_RS22020	4073904..4074959	-	1056	WP_001226164.1	DNA polymerase IV	-
CR536_RS22025	4075030..4075815	-	786	WP_162541364.1	putative lateral flagellar export/assembly protein LafU	-
CR536_RS22030	4075787..4077499	+	1713	Protein_4006	flagellar biosynthesis protein FlhA	-
CR536_RS22040	4077925..4078422	-	498	WP_033802985.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T86853 WP_001263493.1 NZ_CP024134:c4073556-4073158 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T86853 NZ_CP024134:c4073556-4073158 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT86853 WP_000554757.1 NZ_CP024134:c4073852-4073559 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT86853 NZ_CP024134:c4073852-4073559 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6