Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1927115..1927617 Replicon chromosome
Accession NZ_CP024134
Organism Escherichia coli strain 14EC017

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CR536_RS10175 Protein ID WP_000767829.1
Coordinates 1927363..1927617 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CR536_RS10170 Protein ID WP_001259255.1
Coordinates 1927115..1927366 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR536_RS10140 1922301..1923368 - 1068 WP_021570401.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CR536_RS10145 1923368..1924438 - 1071 WP_021570402.1 histidinol-phosphate transaminase -
CR536_RS10150 1924435..1925739 - 1305 WP_021570403.1 histidinol dehydrogenase -
CR536_RS10155 1925745..1926644 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CR536_RS10170 1927115..1927366 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CR536_RS10175 1927363..1927617 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CR536_RS10180 1927700..1928524 + 825 WP_021570404.1 SDR family oxidoreductase -
CR536_RS10185 1928570..1929499 + 930 WP_000803351.1 LysR family transcriptional regulator -
CR536_RS10190 1929714..1929776 + 63 WP_010723108.1 membrane protein YoeI -
CR536_RS10195 1929766..1931124 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CR536_RS10200 1931303..1932361 + 1059 WP_024207725.1 transport protein YeeE -
CR536_RS10205 1932375..1932602 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86840 WP_000767829.1 NZ_CP024134:1927363-1927617 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86840 NZ_CP024134:1927363-1927617 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86840 WP_001259255.1 NZ_CP024134:1927115-1927366 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86840 NZ_CP024134:1927115-1927366 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References