Detailed information of TA system TA086831

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64323..64592
• Replicon: plasmid p14EC007b
• Accession: NZ_CP024133
• Organism: Escherichia coli strain 14EC007

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CR535_RS27860
• Protein ID: WP_001312861.1
• Coordinates: 64434..64592 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	64323..64388 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR535_RS27830	60116..60643	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
CR535_RS27835	60699..60932	+	234	WP_000006004.1	DUF905 domain-containing protein	-
CR535_RS27840	60991..62949	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
CR535_RS27845	63004..63438	+	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
CR535_RS27850	63435..64154	+	720	WP_001276228.1	plasmid SOS inhibition protein A	-
CR535_RS27855	64166..64354	-	189	WP_001299721.1	hypothetical protein	-
-	64166..64390	+	225	NuclAT_0	-	-
-	64166..64390	+	225	NuclAT_0	-	-
-	64166..64390	+	225	NuclAT_0	-	-
-	64166..64390	+	225	NuclAT_0	-	-
-	64323..64388	+	66	-	-	Antitoxin
CR535_RS27860	64434..64592	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CR535_RS27880	65514..65801	+	288	WP_000107535.1	hypothetical protein	-
CR535_RS27885	65921..66742	+	822	WP_001241365.1	DUF945 domain-containing protein	-
CR535_RS27890	67039..67641	-	603	WP_000243705.1	transglycosylase SLT domain-containing protein	-
CR535_RS27895	67962..68345	+	384	WP_001151524.1	relaxosome protein TraM	-
CR535_RS27900	68532..69221	+	690	WP_000283379.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / sul3 / blaTEM-1B / qnrS1 / dfrA12 / aadA2 / tet(A)	stcE / exeE / exeG	1..190293	190293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86831 WP_001312861.1 NZ_CP024133:64434-64592 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86831 NZ_CP024133:64434-64592 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT86831 NZ_CP024133:64323-64388 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin