Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3981733..3982274 Replicon chromosome
Accession NZ_CP024131
Organism Escherichia coli strain 14EC007

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0K4UG80
Locus tag CR535_RS21430 Protein ID WP_033802988.1
Coordinates 3981996..3982274 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A0K5TST2
Locus tag CR535_RS21425 Protein ID WP_033802987.1
Coordinates 3981733..3981993 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR535_RS21400 3977206..3977991 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
CR535_RS21405 3977963..3979675 + 1713 Protein_3897 flagellar biosynthesis protein FlhA -
CR535_RS21415 3980101..3980598 - 498 WP_033802985.1 REP-associated tyrosine transposase RayT -
CR535_RS21420 3980774..3981523 - 750 WP_033802986.1 C40 family peptidase -
CR535_RS21425 3981733..3981993 + 261 WP_033802987.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CR535_RS21430 3981996..3982274 + 279 WP_033802988.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CR535_RS21435 3982430..3983170 + 741 WP_001225679.1 murein L,D-transpeptidase -
CR535_RS21440 3983141..3983908 - 768 WP_000333380.1 class II glutamine amidotransferase -
CR535_RS21445 3984114..3984692 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10828.65 Da        Isoelectric Point: 10.0702

>T86825 WP_033802988.1 NZ_CP024131:3981996-3982274 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAVLFG

Download         Length: 279 bp

>T86825 NZ_CP024131:3981996-3982274 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATAAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTACAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGTGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9390.79 Da        Isoelectric Point: 5.8129

>AT86825 WP_033802987.1 NZ_CP024131:3981733-3981993 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKNADDL
FDKLGI

Download         Length: 261 bp

>AT86825 NZ_CP024131:3981733-3981993 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAAACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4UG80

Antitoxin

Source ID Structure
AlphaFold DB A0A0K5TST2

References