Detailed information of TA system TA086824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3975334..3976028
• Replicon: chromosome
• Accession: NZ_CP024131
• Organism: Escherichia coli strain 14EC007

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CR535_RS21385
• Protein ID: WP_001263493.1
• Coordinates: 3975334..3975732 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CR535_RS21390
• Protein ID: WP_000554757.1
• Coordinates: 3975735..3976028 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR535_RS21355	3970334..3971578	-	1245	WP_000189541.1	esterase FrsA	-
-	3970994..3971074	-	81	NuclAT_9	-	-
-	3970994..3971074	-	81	NuclAT_9	-	-
-	3970994..3971074	-	81	NuclAT_9	-	-
-	3970994..3971074	-	81	NuclAT_9	-	-
CR535_RS21360	3971670..3972128	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CR535_RS21365	3972389..3973846	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CR535_RS21370	3973903..3974424	-	522	Protein_3890	peptide chain release factor H	-
CR535_RS21375	3974423..3974626	-	204	Protein_3891	RNA ligase RtcB family protein	-
CR535_RS21380	3974872..3975324	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CR535_RS21385	3975334..3975732	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CR535_RS21390	3975735..3976028	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CR535_RS21395	3976080..3977135	-	1056	WP_001226164.1	DNA polymerase IV	-
CR535_RS21400	3977206..3977991	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
CR535_RS21405	3977963..3979675	+	1713	Protein_3897	flagellar biosynthesis protein FlhA	-
CR535_RS21415	3980101..3980598	-	498	WP_033802985.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T86824 WP_001263493.1 NZ_CP024131:c3975732-3975334 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T86824 NZ_CP024131:c3975732-3975334 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT86824 WP_000554757.1 NZ_CP024131:c3976028-3975735 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT86824 NZ_CP024131:c3976028-3975735 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6