Detailed information of TA system TA086803

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93221..93647
• Replicon: plasmid p14EC001b
• Accession: NZ_CP024129
• Organism: Escherichia coli strain 14EC001

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CR534_RS27980
• Protein ID: WP_001312861.1
• Coordinates: 93221..93379 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	93423..93647 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR534_RS27940	88592..89281	-	690	WP_000283379.1	conjugal transfer transcriptional regulator TraJ	-
CR534_RS27945	89468..89851	-	384	WP_001151524.1	relaxosome protein TraM	-
CR534_RS27950	90172..90774	+	603	WP_000243705.1	transglycosylase SLT domain-containing protein	-
CR534_RS27955	91071..91892	-	822	WP_001241365.1	DUF945 domain-containing protein	-
CR534_RS27960	92012..92299	-	288	WP_000107535.1	hypothetical protein	-
CR534_RS29425	92324..92530	-	207	WP_033804333.1	hypothetical protein	-
CR534_RS29585	92771..92926	-	156	WP_213080977.1	hypothetical protein	-
CR534_RS27980	93221..93379	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	93423..93647	-	225	NuclAT_0	-	Antitoxin
-	93423..93647	-	225	NuclAT_0	-	Antitoxin
-	93423..93647	-	225	NuclAT_0	-	Antitoxin
-	93423..93647	-	225	NuclAT_0	-	Antitoxin
CR534_RS29175	93459..93647	+	189	WP_001299721.1	hypothetical protein	-
CR534_RS27985	93659..94378	-	720	WP_001276240.1	plasmid SOS inhibition protein A	-
CR534_RS27990	94375..94809	-	435	WP_101981708.1	conjugation system SOS inhibitor PsiB	-
CR534_RS27995	94864..96822	-	1959	WP_063072993.1	ParB/RepB/Spo0J family partition protein	-
CR534_RS28000	96887..97120	-	234	WP_000006030.1	DUF905 family protein	-
CR534_RS28005	97182..97634	-	453	WP_000290842.1	single-stranded DNA-binding protein	-
CR534_RS28025	98107..98364	-	258	WP_072193410.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B)	stcE / exeE / exeG / stcE / stcE / stcE / stcE / exeE / exeG	1..123884	123884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T86803 WP_001312861.1 NZ_CP024129:c93379-93221 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86803 NZ_CP024129:c93379-93221 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT86803 NZ_CP024129:c93647-93423 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin