Detailed information of TA system TA086800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4054971..4055665
• Replicon: chromosome
• Accession: NZ_CP024127
• Organism: Escherichia coli strain 14EC001

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CR534_RS21920
• Protein ID: WP_001263493.1
• Coordinates: 4055267..4055665 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CR534_RS21915
• Protein ID: WP_000554757.1
• Coordinates: 4054971..4055264 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR534_RS21895	4050603..4051100	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
CR534_RS21900	4051324..4053036	-	1713	Protein_3980	flagellar biosynthesis protein FlhA	-
CR534_RS21905	4053008..4053793	+	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
CR534_RS21910	4053864..4054919	+	1056	WP_001226164.1	DNA polymerase IV	-
CR534_RS21915	4054971..4055264	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CR534_RS21920	4055267..4055665	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CR534_RS21925	4055675..4056127	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CR534_RS21930	4056373..4056576	+	204	Protein_3986	RNA ligase RtcB family protein	-
CR534_RS21935	4056575..4057096	+	522	Protein_3987	peptide chain release factor H	-
CR534_RS21940	4057153..4058610	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CR534_RS21945	4058871..4059329	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CR534_RS21950	4059421..4060665	+	1245	WP_000189541.1	esterase FrsA	-
-	4059925..4060005	+	81	NuclAT_6	-	-
-	4059925..4060005	+	81	NuclAT_6	-	-
-	4059925..4060005	+	81	NuclAT_6	-	-
-	4059925..4060005	+	81	NuclAT_6	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T86800 WP_001263493.1 NZ_CP024127:4055267-4055665 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T86800 NZ_CP024127:4055267-4055665 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT86800 WP_000554757.1 NZ_CP024127:4054971-4055264 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT86800 NZ_CP024127:4054971-4055264 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6