Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4048927..4049468 Replicon chromosome
Accession NZ_CP024127
Organism Escherichia coli strain 14EC001

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CR534_RS21880 Protein ID WP_000615976.1
Coordinates 4048927..4049205 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CR534_RS21885 Protein ID WP_000729704.1
Coordinates 4049208..4049468 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR534_RS21865 4046509..4047087 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CR534_RS21870 4047293..4048060 + 768 WP_000333379.1 class II glutamine amidotransferase -
CR534_RS21875 4048031..4048771 - 741 WP_001225679.1 murein L,D-transpeptidase -
CR534_RS21880 4048927..4049205 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CR534_RS21885 4049208..4049468 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CR534_RS21890 4049654..4050427 + 774 WP_015675129.1 C40 family peptidase -
CR534_RS21895 4050603..4051100 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
CR534_RS21900 4051324..4053036 - 1713 Protein_3980 flagellar biosynthesis protein FlhA -
CR534_RS21905 4053008..4053793 + 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T86799 WP_000615976.1 NZ_CP024127:c4049205-4048927 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T86799 NZ_CP024127:c4049205-4048927 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT86799 WP_000729704.1 NZ_CP024127:c4049468-4049208 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT86799 NZ_CP024127:c4049468-4049208 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References