Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1857813..1858315 Replicon chromosome
Accession NZ_CP024127
Organism Escherichia coli strain 14EC001

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CR534_RS09720 Protein ID WP_000767829.1
Coordinates 1858061..1858315 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CR534_RS09715 Protein ID WP_001259255.1
Coordinates 1857813..1858064 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR534_RS09685 1852991..1854058 - 1068 WP_023147847.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CR534_RS09690 1854058..1855128 - 1071 WP_097514097.1 histidinol-phosphate transaminase -
CR534_RS09695 1855125..1856429 - 1305 WP_001515511.1 histidinol dehydrogenase -
CR534_RS09700 1856435..1857334 - 900 WP_089518536.1 ATP phosphoribosyltransferase -
CR534_RS09710 1857480..1857530 - 51 WP_001364200.1 his operon leader peptide -
CR534_RS09715 1857813..1858064 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CR534_RS09720 1858061..1858315 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CR534_RS09725 1858398..1859222 + 825 WP_000754749.1 SDR family oxidoreductase -
CR534_RS09730 1859268..1860197 + 930 WP_000803351.1 LysR family transcriptional regulator -
CR534_RS09735 1860412..1860474 + 63 WP_010723108.1 membrane protein YoeI -
CR534_RS09740 1860464..1861822 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CR534_RS09745 1862001..1863059 + 1059 WP_000492315.1 transport protein YeeE -
CR534_RS09750 1863073..1863300 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86785 WP_000767829.1 NZ_CP024127:1858061-1858315 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86785 NZ_CP024127:1858061-1858315 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86785 WP_001259255.1 NZ_CP024127:1857813-1858064 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86785 NZ_CP024127:1857813-1858064 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References