Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1813297..1813799 Replicon chromosome
Accession NZ_CP024090
Organism Escherichia coli strain WG5

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CSR56_RS09040 Protein ID WP_000767829.1
Coordinates 1813545..1813799 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CSR56_RS09035 Protein ID WP_001259255.1
Coordinates 1813297..1813548 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSR56_RS09000 1808475..1809542 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CSR56_RS09005 1809542..1810612 - 1071 WP_000108940.1 histidinol-phosphate transaminase -
CSR56_RS09010 1810609..1811913 - 1305 WP_001317939.1 histidinol dehydrogenase -
CSR56_RS09015 1811919..1812818 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CSR56_RS09025 1812964..1813014 - 51 WP_001364200.1 his operon leader peptide -
CSR56_RS09035 1813297..1813548 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CSR56_RS09040 1813545..1813799 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CSR56_RS09045 1813882..1814706 + 825 WP_000754737.1 SDR family oxidoreductase -
CSR56_RS09050 1814752..1815681 + 930 WP_081508817.1 LysR family transcriptional regulator -
CSR56_RS09055 1815896..1815958 + 63 WP_010723108.1 membrane protein YoeI -
CSR56_RS09060 1815948..1817306 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CSR56_RS09065 1817485..1818543 + 1059 WP_000492305.1 transport protein YeeE -
CSR56_RS09070 1818557..1818784 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86694 WP_000767829.1 NZ_CP024090:1813545-1813799 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86694 NZ_CP024090:1813545-1813799 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86694 WP_001259255.1 NZ_CP024090:1813297-1813548 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86694 NZ_CP024090:1813297-1813548 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References