Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1314110..1314696 Replicon chromosome
Accession NZ_CP024085
Organism Pseudomonas putida strain E41

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag CR511_RS05955 Protein ID WP_099453131.1
Coordinates 1314418..1314696 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag CR511_RS05950 Protein ID WP_099453130.1
Coordinates 1314110..1314409 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CR511_RS05925 1310052..1310861 + 810 WP_027595781.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -
CR511_RS05930 1310871..1311281 - 411 WP_099453128.1 SufE family protein -
CR511_RS05935 1311278..1312483 - 1206 WP_099453129.1 cysteine desulfurase -
CR511_RS05940 1312562..1313596 - 1035 WP_027595784.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
CR511_RS05945 1313638..1313985 - 348 WP_027595785.1 ArsC family reductase -
CR511_RS05950 1314110..1314409 - 300 WP_099453130.1 HigA family addiction module antidote protein Antitoxin
CR511_RS05955 1314418..1314696 - 279 WP_099453131.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CR511_RS05960 1314939..1316585 + 1647 WP_085272745.1 Na+/H+ antiporter -
CR511_RS05965 1316851..1318047 - 1197 WP_099453132.1 succinyldiaminopimelate transaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10491.80 Da        Isoelectric Point: 7.1347

>T86681 WP_099453131.1 NZ_CP024085:c1314696-1314418 [Pseudomonas putida]
MIQAFSCADTEALFTTGKTRRWSDIKSVAERKLTMLDAATELRDLRSPPGNRLEQLSGNRAGQHSIRVNDQWRVCFIWTE
NGPANVEIVDYH

Download         Length: 279 bp

>T86681 NZ_CP024085:c1314696-1314418 [Pseudomonas putida]
ATGATTCAAGCCTTCAGCTGTGCTGACACGGAAGCACTCTTCACGACTGGAAAGACACGGCGTTGGTCAGATATCAAATC
TGTCGCAGAGCGCAAATTGACCATGCTTGATGCTGCCACAGAACTGCGCGACCTTCGCTCTCCTCCGGGCAATCGCCTGG
AACAGTTGAGCGGTAATCGCGCCGGCCAACACAGCATACGAGTCAATGATCAGTGGCGCGTGTGCTTTATCTGGACGGAA
AACGGTCCGGCAAACGTTGAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10901.60 Da        Isoelectric Point: 5.6714

>AT86681 WP_099453130.1 NZ_CP024085:c1314409-1314110 [Pseudomonas putida]
MLKNGMRPIHPGEVLREDFQKEMGFSAAALARALGVATPTVNNILRERGGISADMALRLAICLDTTPEFWLNLQIAFDLR
TAEQQHGDEIIASVQRLVA

Download         Length: 300 bp

>AT86681 NZ_CP024085:c1314409-1314110 [Pseudomonas putida]
ATGCTCAAGAATGGCATGCGTCCAATACATCCTGGTGAAGTTCTGCGCGAGGACTTCCAGAAAGAAATGGGGTTCAGTGC
CGCTGCTCTGGCGCGGGCATTGGGTGTTGCGACGCCAACGGTCAACAACATCCTGCGCGAGCGTGGCGGCATTTCCGCCG
ACATGGCACTGCGCCTGGCCATCTGCCTGGATACCACACCGGAGTTCTGGCTCAACCTGCAAATCGCCTTCGATTTGCGC
ACAGCCGAACAGCAACATGGCGATGAGATCATCGCATCCGTGCAGCGCTTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References