Detailed information of TA system TA086657

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4448663..4449075
• Replicon: chromosome
• Accession: NZ_CP024056
• Organism: Escherichia coli strain SMN197SH3

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0B1K116
• Locus tag: CRT55_RS25250
• Protein ID: WP_000132623.1
• Coordinates: 4448663..4449004 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4448999..4449075 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT55_RS25235	4444672..4446084	-	1413	WP_000199311.1	PLP-dependent aminotransferase family protein	-
CRT55_RS25240	4446261..4446425	+	165	WP_000394278.1	DUF1127 domain-containing protein	-
CRT55_RS25245	4446523..4448616	+	2094	WP_062881594.1	DUF262 domain-containing protein	-
CRT55_RS25250	4448663..4449004	-	342	WP_000132623.1	endoribonuclease SymE	Toxin
-	4448999..4449075	+	77	NuclAT_27	-	Antitoxin
-	4448999..4449075	+	77	NuclAT_27	-	Antitoxin
-	4448999..4449075	+	77	NuclAT_27	-	Antitoxin
-	4448999..4449075	+	77	NuclAT_27	-	Antitoxin
-	4448999..4449075	+	77	NuclAT_28	-	Antitoxin
-	4448999..4449075	+	77	NuclAT_28	-	Antitoxin
-	4448999..4449075	+	77	NuclAT_28	-	Antitoxin
-	4448999..4449075	+	77	NuclAT_28	-	Antitoxin
CRT55_RS25260	4449993..4453256	-	3264	WP_062881360.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12323.21 Da
• Isoelectric Point: 9.5818

>T86657 WP_000132623.1 NZ_CP024056:c4449004-4448663 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMKGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVREFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T86657 NZ_CP024056:c4449004-4448663 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTATCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCTA
CCGGCACGGCGGTAGATGTCAAAGTGATGAAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAG
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCGGGAATTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT86657 NZ_CP024056:4448999-4449075 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0B1K116

Antitoxin