Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4105743..4106284 Replicon chromosome
Accession NZ_CP024056
Organism Escherichia coli strain SMN197SH3

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag CRT55_RS23570 Protein ID WP_000615979.1
Coordinates 4106006..4106284 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CRT55_RS23565 Protein ID WP_000729704.1
Coordinates 4105743..4106003 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRT55_RS23535 4101989..4102207 - 219 Protein_4160 GNAT family N-acetyltransferase -
CRT55_RS23540 4102266..4102960 + 695 WP_085949012.1 IS1 family transposase -
CRT55_RS23545 4102976..4103215 - 240 Protein_4162 GNAT family N-acetyltransferase -
CRT55_RS23550 4103212..4104267 - 1056 WP_001226164.1 DNA polymerase IV -
CRT55_RS23555 4104338..4105123 - 786 WP_062882563.1 putative lateral flagellar export/assembly protein LafU -
CRT55_RS23560 4105095..4105456 + 362 Protein_4165 FHIPEP family type III secretion protein -
CRT55_RS30485 4105447..4105557 - 111 Protein_4166 peptidoglycan endopeptidase -
CRT55_RS23565 4105743..4106003 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CRT55_RS23570 4106006..4106284 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CRT55_RS23575 4106440..4107180 + 741 WP_001225679.1 murein L,D-transpeptidase -
CRT55_RS23580 4107151..4107918 - 768 WP_001365365.1 class II glutamine amidotransferase -
CRT55_RS23585 4108124..4108702 - 579 WP_062882565.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T86656 WP_000615979.1 NZ_CP024056:4106006-4106284 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T86656 NZ_CP024056:4106006-4106284 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT86656 WP_000729704.1 NZ_CP024056:4105743-4106003 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT86656 NZ_CP024056:4105743-4106003 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References