Detailed information of TA system TA086630

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72278..72532
• Replicon: plasmid pO177A3
• Accession: NZ_CP024055
• Organism: Escherichia coli strain SMN197SH3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CRT55_RS00805
• Protein ID: WP_001351576.1
• Coordinates: 72278..72484 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	72471..72532 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRT55_RS00765	68635..68910	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CRT55_RS00770	68910..69194	-	285	WP_062881718.1	ribbon-helix-helix domain-containing protein	-
CRT55_RS00780	70106..70963	-	858	WP_096851680.1	incFII family plasmid replication initiator RepA	-
CRT55_RS00785	70956..71438	-	483	WP_001523473.1	hypothetical protein	-
CRT55_RS00790	71431..71505	-	75	WP_001442103.1	RepA leader peptide Tap	-
CRT55_RS00800	71737..71994	-	258	WP_000083845.1	replication regulatory protein RepA	-
CRT55_RS00805	72278..72484	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	72471..72532	+	62	NuclAT_0	-	Antitoxin
-	72471..72532	+	62	NuclAT_0	-	Antitoxin
-	72471..72532	+	62	NuclAT_0	-	Antitoxin
-	72471..72532	+	62	NuclAT_0	-	Antitoxin
CRT55_RS30290	72788..72862	-	75	Protein_74	endonuclease	-
CRT55_RS00815	73108..73320	-	213	WP_001299730.1	ANR family transcriptional regulator	-
CRT55_RS00820	73456..74016	-	561	WP_062881716.1	fertility inhibition protein FinO	-
CRT55_RS00825	74071..74817	-	747	WP_062881715.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / espP / hlyC / hlyA / hlyB	1..88839	88839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T86630 WP_001351576.1 NZ_CP024055:c72484-72278 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T86630 NZ_CP024055:c72484-72278 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT86630 NZ_CP024055:72471-72532 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin