Detailed information of TA system TA086618

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93795..94216
• Replicon: plasmid pMR0617ndm
• Accession: NZ_CP024039
• Organism: Klebsiella pneumoniae strain QS17-0029

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: CR230_RS27530
• Protein ID: WP_001302184.1
• Coordinates: 93795..93953 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	94018..94216 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR230_RS27495	88859..89074	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
CR230_RS27500	89210..89857	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
CR230_RS27505	90048..90431	-	384	WP_001063020.1	relaxosome protein TraM	-
CR230_RS27510	90801..91373	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
CR230_RS27515	91669..92490	-	822	WP_001234465.1	DUF945 domain-containing protein	-
CR230_RS27520	92609..92896	-	288	WP_000107242.1	hypothetical protein	-
CR230_RS29680	92921..93127	-	207	WP_000275854.1	hypothetical protein	-
CR230_RS27530	93795..93953	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	94018..94216	-	199	NuclAT_0	-	Antitoxin
-	94018..94216	-	199	NuclAT_0	-	Antitoxin
-	94018..94216	-	199	NuclAT_0	-	Antitoxin
-	94018..94216	-	199	NuclAT_0	-	Antitoxin
CR230_RS27535	94445..94762	+	318	Protein_116	IS110 family transposase	-
CR230_RS27540	95051..95302	-	252	Protein_117	iron-containing alcohol dehydrogenase	-
CR230_RS27545	95387..96010	-	624	WP_000912556.1	potassium channel family protein	-
CR230_RS27550	96026..96719	-	694	Protein_119	IS1 family transposase	-
CR230_RS27560	96776..96880	+	105	Protein_120	thiosulfate reductase cytochrome B subunit	-
CR230_RS27570	97126..98520	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1	-	1..125285	125285
	-	flank	IS/Tn	-	-	94394..94762	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T86618 WP_001302184.1 NZ_CP024039:c93953-93795 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T86618 NZ_CP024039:c93953-93795 [Klebsiella pneumoniae]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT86618 NZ_CP024039:c94216-94018 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin