Detailed information of TA system TA086616

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 77048..77302
• Replicon: plasmid pMR0617ndm
• Accession: NZ_CP024039
• Organism: Klebsiella pneumoniae strain QS17-0029

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CR230_RS27385
• Protein ID: WP_015387399.1
• Coordinates: 77048..77254 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	77241..77302 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR230_RS27350	72295..73206	-	912	WP_000440183.1	carbamate kinase	-
CR230_RS27355	73217..74437	-	1221	WP_000410951.1	arginine deiminase	-
CR230_RS27360	75330..75812	+	483	Protein_84	VENN motif pre-toxin domain-containing protein	-
CR230_RS27365	75758..76204	-	447	Protein_85	incFII family plasmid replication initiator RepA	-
CR230_RS27370	76197..76271	-	75	WP_001365705.1	RepA leader peptide Tap	-
CR230_RS27380	76507..76764	-	258	WP_000083833.1	replication regulatory protein RepA	-
CR230_RS27385	77048..77254	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	77241..77302	+	62	NuclAT_1	-	Antitoxin
-	77241..77302	+	62	NuclAT_1	-	Antitoxin
-	77241..77302	+	62	NuclAT_1	-	Antitoxin
-	77241..77302	+	62	NuclAT_1	-	Antitoxin
CR230_RS27395	77441..77623	-	183	WP_000968309.1	hypothetical protein	-
CR230_RS27405	77724..78426	+	703	Protein_90	IS1 family transposase	-
CR230_RS27415	78727..78939	-	213	WP_005012601.1	hypothetical protein	-
CR230_RS27420	79072..79632	-	561	WP_000139363.1	fertility inhibition protein FinO	-
CR230_RS27425	79687..80433	-	747	WP_094083868.1	conjugal transfer pilus acetylase TraX	-
CR230_RS27430	80453..81643	-	1191	Protein_94	conjugative transfer relaxase/helicase TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-1	-	1..125285	125285

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T86616 WP_015387399.1 NZ_CP024039:c77254-77048 [Klebsiella pneumoniae]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T86616 NZ_CP024039:c77254-77048 [Klebsiella pneumoniae]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT86616 NZ_CP024039:77241-77302 [Klebsiella pneumoniae]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin