Detailed information of TA system TA086579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3882780..3883375
• Replicon: chromosome
• Accession: NZ_CP024024
• Organism: Pseudomonas aeruginosa strain PARM801

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: BBX44_RS18430
• Protein ID: WP_003113526.1
• Coordinates: 3883097..3883375 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBX44_RS18425
• Protein ID: WP_003099268.1
• Coordinates: 3882780..3883085 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBX44_RS18400	3878112..3878402	-	291	WP_023088865.1	DUF5447 family protein	-
BBX44_RS18405	3878406..3878618	-	213	WP_121496270.1	hypothetical protein	-
BBX44_RS18410	3878614..3878886	-	273	WP_003115921.1	hypothetical protein	-
BBX44_RS18415	3879333..3879668	+	336	WP_121496269.1	hypothetical protein	-
BBX44_RS18420	3879742..3882102	-	2361	WP_162950028.1	ATP-binding protein	-
BBX44_RS18425	3882780..3883085	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
BBX44_RS18430	3883097..3883375	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBX44_RS18435	3883704..3885932	+	2229	WP_003099265.1	TonB-dependent receptor	-
BBX44_RS18440	3886002..3886649	-	648	WP_003095021.1	carbonate dehydratase	-
BBX44_RS18445	3886711..3887949	-	1239	WP_047689994.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T86579 WP_003113526.1 NZ_CP024024:c3883375-3883097 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T86579 NZ_CP024024:c3883375-3883097 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT86579 WP_003099268.1 NZ_CP024024:c3883085-3882780 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT86579 NZ_CP024024:c3883085-3882780 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3