Detailed information of TA system TA086566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1677545..1678206
• Replicon: chromosome
• Accession: NZ_CP023994
• Organism: Aurantimicrobium sp. MWH-Mo1

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: AURMO_RS08440
• Protein ID: WP_110234738.1
• Coordinates: 1677847..1678206 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: AURMO_RS08435
• Protein ID: WP_110234737.1
• Coordinates: 1677545..1677847 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AURMO_RS08420	1672969..1674840	-	1872	WP_110234734.1	molecular chaperone DnaK	-
AURMO_RS08425	1675061..1676437	+	1377	WP_110234735.1	MFS transporter	-
AURMO_RS08430	1676434..1677528	-	1095	WP_110234736.1	NADH:flavin oxidoreductase/NADH oxidase	-
AURMO_RS08435	1677545..1677847	-	303	WP_110234737.1	helix-turn-helix transcriptional regulator	Antitoxin
AURMO_RS08440	1677847..1678206	-	360	WP_110234738.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AURMO_RS08445	1678347..1679240	-	894	WP_110234739.1	prenyltransferase	-
AURMO_RS08450	1679225..1679548	-	324	WP_110234740.1	lycopene cyclase domain-containing protein	-
AURMO_RS08455	1679545..1679874	-	330	WP_110234741.1	lycopene cyclase domain-containing protein	-
AURMO_RS08460	1679880..1681493	-	1614	WP_110234742.1	phytoene desaturase	-
AURMO_RS08465	1681490..1682404	-	915	WP_110234743.1	squalene/phytoene synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (19-91)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13640.61 Da
• Isoelectric Point: 9.9595

>T86566 WP_110234738.1 NZ_CP023994:c1678206-1677847 [Aurantimicrobium sp. MWH-Mo1]
MVTWEVDVELIKIWFSRLDINSRAQVQAAIELLESEGPQLGRPLVDSVNGSAHKNMKELRPGSSGRSELRILFVFDPDRK
AIMLVAGDKSGTWSKWYRTSIRKADQLYTEHVEKRKQAQ

Nucleotide

• Length: 360 bp

>T86566 NZ_CP023994:c1678206-1677847 [Aurantimicrobium sp. MWH-Mo1]
ATGGTGACGTGGGAAGTTGACGTTGAGTTGATCAAGATCTGGTTCTCCAGGCTGGATATCAACTCAAGGGCACAGGTTCA
GGCAGCAATTGAGCTACTTGAGTCCGAAGGTCCACAGCTAGGCCGCCCGCTTGTTGATTCTGTCAACGGATCAGCACATA
AGAACATGAAAGAACTTCGACCAGGGTCATCTGGCCGGAGTGAACTTAGAATCTTGTTTGTTTTTGATCCAGACCGAAAA
GCAATCATGTTGGTCGCAGGTGACAAGTCGGGAACATGGTCAAAGTGGTACCGAACCAGCATTCGGAAAGCTGACCAGCT
ATATACGGAACATGTAGAGAAAAGAAAGCAGGCACAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11565.27 Da
• Isoelectric Point: 7.3182

>AT86566 WP_110234737.1 NZ_CP023994:c1677847-1677545 [Aurantimicrobium sp. MWH-Mo1]
MLSPEQLFAEYPSDPAQVEAHKKRMMAEARAYRLKELRKELQITQVELAQQLHVSQNRISRIEHGDIEKSQVDTLRKYVE
AMGGTLRIDVEVDGKKFQLA

Nucleotide

• Length: 303 bp

>AT86566 NZ_CP023994:c1677847-1677545 [Aurantimicrobium sp. MWH-Mo1]
ATGTTGAGTCCAGAGCAACTATTTGCTGAGTACCCCTCTGACCCTGCTCAGGTTGAAGCGCATAAGAAGCGCATGATGGC
GGAAGCGCGTGCATACCGTCTCAAGGAACTCCGCAAAGAACTTCAGATAACTCAGGTTGAACTTGCACAACAGTTACACG
TTAGCCAGAACCGCATCTCTCGAATTGAACACGGTGACATAGAGAAGTCTCAGGTTGATACGTTGCGCAAATATGTGGAG
GCCATGGGTGGAACCCTTCGCATCGACGTTGAAGTGGATGGCAAGAAGTTTCAACTGGCTTAG