Detailed information of TA system TA086561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 166480..167207
• Replicon: plasmid pX39-1
• Accession: NZ_CP023978
• Organism: Klebsiella variicola strain X39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: BC497_RS29165
• Protein ID: WP_011251285.1
• Coordinates: 166480..166791 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BC497_RS29170
• Protein ID: WP_011251286.1
• Coordinates: 166788..167207 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC497_RS29135	162177..162874	-	698	Protein_168	IS1 family transposase	-
BC497_RS29140	163077..164045	-	969	WP_162893604.1	IS5 family transposase	-
BC497_RS29145	164086..164990	-	905	Protein_170	IS66 family transposase	-
BC497_RS29150	165113..165388	-	276	WP_085163641.1	IS66 family insertion sequence element accessory protein TnpB	-
BC497_RS29155	165385..165723	-	339	WP_023288266.1	IS66 family insertion sequence hypothetical protein	-
BC497_RS29160	165817..166275	+	459	Protein_173	DDE-type integrase/transposase/recombinase	-
BC497_RS29165	166480..166791	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
BC497_RS29170	166788..167207	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
BC497_RS29180	167354..168322	+	969	WP_162893605.1	IS5 family transposase	-
BC497_RS29185	168379..168600	-	222	Protein_177	transposase	-
BC497_RS29190	168628..169325	-	698	Protein_178	IS1 family transposase	-
BC497_RS29195	169517..170542	-	1026	WP_001101446.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla / mrkA / mrkB / mrkC / mrkF / mrkJ	1..213341	213341
	-	inside	IScluster/Tn	-	-	148128..170542	22414

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T86561 WP_011251285.1 NZ_CP023978:166480-166791 [Klebsiella variicola]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T86561 NZ_CP023978:166480-166791 [Klebsiella variicola]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT86561 WP_011251286.1 NZ_CP023978:166788-167207 [Klebsiella variicola]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT86561 NZ_CP023978:166788-167207 [Klebsiella variicola]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1