Detailed information of TA system TA086558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 58121..58848
• Replicon: plasmid pX39-1
• Accession: NZ_CP023978
• Organism: Klebsiella variicola strain X39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: BC497_RS28530
• Protein ID: WP_000558568.1
• Coordinates: 58121..58432 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: BC497_RS28535
• Protein ID: WP_000990392.1
• Coordinates: 58429..58848 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BC497_RS28500	54368..54616	+	249	WP_087653731.1	hypothetical protein	-
BC497_RS28510	54885..55582	+	698	Protein_60	IS1 family transposase	-
BC497_RS28515	55599..56792	+	1194	Protein_61	iron-sulfur cluster-binding protein	-
BC497_RS28520	56785..57480	+	696	WP_000370412.1	lactate utilization protein C	-
BC497_RS28525	57530..57910	-	381	Protein_63	gluconate permease	-
BC497_RS28530	58121..58432	+	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
BC497_RS28535	58429..58848	+	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
BC497_RS28540	58885..60087	-	1203	WP_087653634.1	phage tail protein	-
BC497_RS28545	60080..60409	-	330	WP_000121260.1	hypothetical protein	-
BC497_RS28550	60406..61065	-	660	WP_000078540.1	hypothetical protein	-
BC497_RS28560	61489..62082	-	594	WP_087653635.1	hypothetical protein	-
BC497_RS28565	62398..62751	+	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
BC497_RS28570	62799..63161	+	363	WP_000783215.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla / mrkA / mrkB / mrkC / mrkF / mrkJ	1..213341	213341
	-	flank	IS/Tn	-	-	55079..55582	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T86558 WP_000558568.1 NZ_CP023978:58121-58432 [Klebsiella variicola]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T86558 NZ_CP023978:58121-58432 [Klebsiella variicola]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT86558 WP_000990392.1 NZ_CP023978:58429-58848 [Klebsiella variicola]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT86558 NZ_CP023978:58429-58848 [Klebsiella variicola]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: V0AHC4