Detailed information of TA system TA086555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1281107..1281874
• Replicon: chromosome
• Accession: NZ_CP023972
• Organism: Pasteurella multocida strain FDAARGOS_385

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J9QLH7
• Locus tag: CRN72_RS06020
• Protein ID: WP_005725755.1
• Coordinates: 1281107..1281433 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CRN72_RS06025
• Protein ID: WP_096743450.1
• Coordinates: 1281548..1281874 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN72_RS05995	1276424..1277110	+	687	WP_061406323.1	hypothetical protein	-
CRN72_RS06000	1277164..1278693	-	1530	WP_096743448.1	YifB family Mg chelatase-like AAA ATPase	-
CRN72_RS06005	1278802..1279419	-	618	WP_005724253.1	ribosome biogenesis GTP-binding protein YihA/YsxC	-
CRN72_RS06010	1279530..1280399	+	870	WP_096743449.1	VirK/YbjX family protein	-
CRN72_RS06015	1280473..1280949	-	477	WP_042742721.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
CRN72_RS06020	1281107..1281433	+	327	WP_005725755.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRN72_RS06025	1281548..1281874	+	327	WP_096743450.1	HigA family addiction module antidote protein	Antitoxin
CRN72_RS06030	1281929..1283449	-	1521	WP_096743451.1	DUF560 domain-containing protein	-
CRN72_RS06035	1283508..1284098	-	591	WP_096744022.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
CRN72_RS11335	1284161..1285177	-	1017	WP_172459827.1	protein PlpE	-
CRN72_RS06045	1285424..1286455	-	1032	WP_096743452.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12892.73 Da
• Isoelectric Point: 9.6705

>T86555 WP_005725755.1 NZ_CP023972:1281107-1281433 [Pasteurella multocida]
MDRVRFNLTEDSFQDKYLYEYFLYGSKHKNIPSDIENTLAHKLDMLDSANTLSDLRSPPGNKLEKLEPKTSGLYSIRVNV
KYRLIFKYKESNFPKITELRLDKHQYRL

Nucleotide

• Length: 327 bp

>T86555 NZ_CP023972:1281107-1281433 [Pasteurella multocida]
ATGGACAGAGTCCGATTTAATCTTACGGAAGATAGCTTCCAAGACAAGTATCTGTATGAGTATTTTTTATATGGAAGTAA
GCATAAAAATATTCCAAGTGATATAGAAAATACGCTAGCGCATAAATTAGATATGTTAGATAGCGCAAATACTTTGAGCG
ATTTGCGAAGTCCACCAGGTAATAAATTGGAAAAATTGGAGCCTAAAACGAGTGGGCTATATTCTATCAGAGTGAATGTG
AAATATCGTTTAATATTTAAATATAAAGAAAGTAATTTTCCAAAAATAACAGAGTTGAGATTAGATAAACATCAATATAG
ATTATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12387.45 Da
• Isoelectric Point: 7.2034

>AT86555 WP_096743450.1 NZ_CP023972:1281548-1281874 [Pasteurella multocida]
MSRVQRKPSTVGDILLDEYLVPLNLKIADLATMLDVHRNTASALVNNNTKLSLEMALKLAKLFNTSPEFWLNLQMKLDLW
EIENNARFQESLKKISPIDQHNLLEKIA

Nucleotide

• Length: 327 bp

>AT86555 NZ_CP023972:1281548-1281874 [Pasteurella multocida]
ATGAGTAGAGTACAAAGAAAGCCATCAACAGTGGGTGATATTTTATTAGATGAATATTTAGTTCCACTAAATTTGAAAAT
TGCAGATTTAGCTACAATGTTAGATGTTCATCGTAATACAGCGAGTGCACTCGTTAATAATAATACTAAATTGTCTTTAG
AAATGGCACTGAAACTAGCAAAACTATTTAATACAAGTCCTGAGTTTTGGTTAAATTTACAAATGAAATTAGACTTATGG
GAAATTGAAAACAATGCTAGGTTTCAAGAAAGTTTAAAGAAAATTTCGCCTATTGATCAGCATAATCTGTTAGAAAAAAT
CGCTTGA

Structures

Toxin

• AlphaFold DB: A0A2J9QLH7