Detailed information of TA system TA086548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 6366241..6366845
• Replicon: chromosome
• Accession: NZ_CP023969
• Organism: Pseudomonas sp. FDAARGOS_380

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CRN80_RS29615
• Protein ID: WP_098952451.1
• Coordinates: 6366241..6366555 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5M8G9A0
• Locus tag: CRN80_RS29620
• Protein ID: WP_056791674.1
• Coordinates: 6366558..6366845 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN80_RS29595	6361306..6362343	-	1038	WP_098952447.1	histone deacetylase family protein	-
CRN80_RS29600	6362475..6363707	-	1233	WP_098952449.1	Zn-dependent hydrolase	-
CRN80_RS29605	6363719..6365035	-	1317	WP_032892903.1	MHS family MFS transporter	-
CRN80_RS29610	6365193..6366134	+	942	WP_044272854.1	LysR family transcriptional regulator	-
CRN80_RS29615	6366241..6366555	+	315	WP_098952451.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CRN80_RS29620	6366558..6366845	+	288	WP_056791674.1	helix-turn-helix domain-containing protein	Antitoxin
CRN80_RS29625	6367090..6368316	+	1227	WP_056791672.1	MFS transporter	-
CRN80_RS29630	6368389..6368643	-	255	WP_098952452.1	DUF3077 domain-containing protein	-
CRN80_RS29640	6369297..6369536	+	240	WP_020301039.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
CRN80_RS29645	6369526..6369819	+	294	WP_032892910.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CRN80_RS29655	6370088..6370801	-	714	WP_027604309.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CRN80_RS29660	6370829..6371587	-	759	WP_003189469.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11692.53 Da
• Isoelectric Point: 9.9127

>T86548 WP_098952451.1 NZ_CP023969:6366241-6366555 [Pseudomonas sp. FDAARGOS_380]
MIFIETSVFTRRVKELIDEDAHMAFQNLLVVNPAAGDVIEGTGGIRKIRVAAKGHGKRGGARVIYYHFVSASQIVLLMIY
PKNEQQDLSADERKSLKAAIEHWR

Nucleotide

• Length: 315 bp

>T86548 NZ_CP023969:6366241-6366555 [Pseudomonas sp. FDAARGOS_380]
ATGATTTTCATAGAAACATCGGTCTTCACCCGTCGCGTTAAAGAATTGATCGACGAGGATGCCCACATGGCCTTTCAGAA
CCTGCTTGTAGTGAATCCCGCTGCCGGCGACGTGATCGAAGGTACGGGTGGCATTCGCAAGATACGAGTAGCAGCCAAGG
GCCATGGAAAACGAGGCGGGGCACGTGTGATCTATTACCACTTCGTCTCGGCATCACAGATTGTTTTACTGATGATCTAT
CCCAAAAATGAACAGCAGGACTTGAGCGCCGACGAGCGCAAGTCACTGAAGGCTGCGATTGAGCACTGGAGATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10773.33 Da
• Isoelectric Point: 6.7281

>AT86548 WP_056791674.1 NZ_CP023969:6366558-6366845 [Pseudomonas sp. FDAARGOS_380]
MSRFFDELMESVQEMNEILSGEREPSREFHIDSMRVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLK
AIHNDPVHVLNALAS

Nucleotide

• Length: 288 bp

>AT86548 NZ_CP023969:6366558-6366845 [Pseudomonas sp. FDAARGOS_380]
ATGAGCAGATTTTTTGATGAATTGATGGAAAGCGTTCAAGAGATGAACGAGATCCTGAGCGGAGAACGGGAGCCTTCTCG
TGAATTCCATATCGACTCAATGCGAGTCAAAGAGATCCGTAAGGCGACAGGGCTGACCCAGGCTAAATTCGCAGCGCTGA
TTGACGTTCAGCTCGGCACGTTACGCAACTGGGAGCAGGGTCGTCGCGAACCTACTGGGCCGGCGAAGGCGTTGTTGAAA
GCTATCCATAATGACCCAGTACATGTACTGAATGCGTTGGCTAGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A5M8G9A0