Detailed information of TA system TA086489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4757056..4757783
• Replicon: chromosome
• Accession: NZ_CP023960
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: CRN68_RS25820
• Protein ID: WP_001521139.1
• Coordinates: 4757056..4757367 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CRN68_RS25825
• Protein ID: WP_000126294.1
• Coordinates: 4757364..4757783 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS25795	4752991..4754700	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
CRN68_RS25800	4754710..4755252	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CRN68_RS25805	4755252..4756019	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
CRN68_RS25810	4756016..4756426	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CRN68_RS25815	4756419..4756889	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CRN68_RS25820	4757056..4757367	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
CRN68_RS25825	4757364..4757783	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CRN68_RS25830	4757862..4759286	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
CRN68_RS25835	4759295..4760752	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CRN68_RS25840	4761012..4762022	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
CRN68_RS25845	4762171..4762698	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T86489 WP_001521139.1 NZ_CP023960:4757056-4757367 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T86489 NZ_CP023960:4757056-4757367 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT86489 WP_000126294.1 NZ_CP023960:4757364-4757783 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT86489 NZ_CP023960:4757364-4757783 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9