Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 4281224..4281917 Replicon chromosome
Accession NZ_CP023960
Organism Escherichia coli strain FDAARGOS_448

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag CRN68_RS23415 Protein ID WP_000415584.1
Coordinates 4281224..4281520 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag CRN68_RS23420 Protein ID WP_000650107.1
Coordinates 4281522..4281917 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN68_RS23375 4276312..4276626 - 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -
CRN68_RS23380 4276657..4277238 - 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
CRN68_RS23390 4277557..4277889 + 333 WP_023149630.1 DUF2645 family protein -
CRN68_RS23395 4277935..4279284 - 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
CRN68_RS23400 4279281..4279940 - 660 WP_001221493.1 two-component system response regulator QseB -
CRN68_RS23405 4280092..4280484 + 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
CRN68_RS23410 4280537..4281019 + 483 WP_000183505.1 transcriptional regulator -
CRN68_RS23415 4281224..4281520 + 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
CRN68_RS23420 4281522..4281917 + 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
CRN68_RS23425 4282050..4283657 + 1608 WP_001295629.1 ABC transporter substrate-binding protein -
CRN68_RS23430 4283795..4286053 + 2259 WP_001281841.1 DNA topoisomerase IV subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T86485 WP_000415584.1 NZ_CP023960:4281224-4281520 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T86485 NZ_CP023960:4281224-4281520 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT86485 WP_000650107.1 NZ_CP023960:4281522-4281917 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT86485 NZ_CP023960:4281522-4281917 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References