Detailed information of TA system TA086483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3338503..3339105
• Replicon: chromosome
• Accession: NZ_CP023960
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CRN68_RS18520
• Protein ID: WP_000897305.1
• Coordinates: 3338794..3339105 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CRN68_RS18515
• Protein ID: WP_000356395.1
• Coordinates: 3338503..3338793 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS18480	3334126..3335028	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CRN68_RS18485	3335025..3335660	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CRN68_RS18490	3335657..3336586	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CRN68_RS18495	3336768..3337010	-	243	WP_021523315.1	hypothetical protein	-
CRN68_RS18500	3337229..3337447	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CRN68_RS18505	3337866..3338144	-	279	WP_001306650.1	hypothetical protein	-
CRN68_RS18510	3338206..3338418	-	213	WP_000197769.1	hypothetical protein	-
CRN68_RS18515	3338503..3338793	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
CRN68_RS18520	3338794..3339105	-	312	WP_000897305.1	hypothetical protein	Toxin
CRN68_RS18525	3339334..3340242	+	909	WP_001306651.1	alpha/beta hydrolase	-
CRN68_RS18535	3340411..3342225	-	1815	WP_020233236.1	hypothetical protein	-
CRN68_RS18540	3342641..3343582	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CRN68_RS18545	3343627..3344064	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T86483 WP_000897305.1 NZ_CP023960:c3339105-3338794 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T86483 NZ_CP023960:c3339105-3338794 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT86483 WP_000356395.1 NZ_CP023960:c3338793-3338503 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT86483 NZ_CP023960:c3338793-3338503 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7