Detailed information of TA system TA086476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2386422..2387116
• Replicon: chromosome
• Accession: NZ_CP023960
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: CRN68_RS13630
• Protein ID: WP_001521903.1
• Coordinates: 2386422..2386820 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: CRN68_RS13635
• Protein ID: WP_000554758.1
• Coordinates: 2386823..2387116 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS13600	2381592..2382836	-	1245	WP_000189541.1	esterase FrsA	-
-	2382252..2382332	-	81	NuclAT_11	-	-
-	2382252..2382332	-	81	NuclAT_11	-	-
-	2382252..2382332	-	81	NuclAT_11	-	-
-	2382252..2382332	-	81	NuclAT_11	-	-
CRN68_RS13605	2382928..2383386	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CRN68_RS13610	2383647..2385104	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
CRN68_RS29470	2385161..2385513	-	353	Protein_2264	peptide chain release factor H	-
CRN68_RS13620	2385509..2385715	-	207	Protein_2265	RtcB family protein	-
CRN68_RS13625	2385960..2386412	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
CRN68_RS13630	2386422..2386820	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CRN68_RS13635	2386823..2387116	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CRN68_RS13640	2387168..2388223	-	1056	WP_001226177.1	DNA polymerase IV	-
CRN68_RS13645	2388294..2389079	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
CRN68_RS13650	2389051..2390763	+	1713	Protein_2271	flagellar biosynthesis protein FlhA	-
CRN68_RS13655	2390842..2391195	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
CRN68_RS13660	2391252..2392001	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2376359..2387116	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T86476 WP_001521903.1 NZ_CP023960:c2386820-2386422 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T86476 NZ_CP023960:c2386820-2386422 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT86476 WP_000554758.1 NZ_CP023960:c2387116-2386823 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT86476 NZ_CP023960:c2387116-2386823 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3