86472

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1559177..1559679 Replicon chromosome
Accession NZ_CP023960
Organism Escherichia coli strain FDAARGOS_448

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CRN68_RS09515 Protein ID WP_000767829.1
Coordinates 1559177..1559431 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CRN68_RS09520 Protein ID WP_001259255.1
Coordinates 1559428..1559679 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRN68_RS09485 1554192..1554419 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CRN68_RS09490 1554433..1555491 - 1059 WP_000492324.1 transport protein YeeE -
CRN68_RS09495 1555670..1557028 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CRN68_RS09500 1557018..1557080 - 63 WP_010723108.1 membrane protein YoeI -
CRN68_RS09505 1557295..1558224 - 930 WP_023154044.1 LysR family transcriptional regulator -
CRN68_RS09510 1558270..1559094 - 825 WP_000754758.1 SDR family oxidoreductase -
CRN68_RS09515 1559177..1559431 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CRN68_RS09520 1559428..1559679 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CRN68_RS09525 1559962..1560012 + 51 WP_001364200.1 his operon leader peptide -
CRN68_RS09535 1560158..1561057 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
CRN68_RS09540 1561063..1562367 + 1305 WP_021523144.1 histidinol dehydrogenase -
CRN68_RS09545 1562364..1563434 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
CRN68_RS09550 1563434..1564501 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T86472 WP_000767829.1 NZ_CP023960:c1559431-1559177 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T86472 NZ_CP023960:c1559431-1559177 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT86472 WP_001259255.1 NZ_CP023960:c1559679-1559428 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT86472 NZ_CP023960:c1559679-1559428 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References