Detailed information of TA system TA086457

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 785176..785547
• Replicon: chromosome
• Accession: NZ_CP023960
• Organism: Escherichia coli strain FDAARGOS_448

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: CRN68_RS05365
• Protein ID: WP_001443846.1
• Coordinates: 785398..785547 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	785176..785354 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CRN68_RS05340	781131..782504	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
CRN68_RS05345	782633..783568	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
CRN68_RS05350	783620..784855	-	1236	WP_000040839.1	site-specific integrase	-
CRN68_RS05355	784857..785072	-	216	WP_000079604.1	excisionase XisR	-
CRN68_RS05360	785172..785360	-	189	WP_001302840.1	DUF1187 family protein	-
-	785176..785354	+	179	NuclAT_0	-	Antitoxin
-	785176..785354	+	179	NuclAT_0	-	Antitoxin
-	785176..785354	+	179	NuclAT_0	-	Antitoxin
-	785176..785354	+	179	NuclAT_0	-	Antitoxin
CRN68_RS05365	785398..785547	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
CRN68_RS05370	785603..786412	-	810	WP_000166313.1	recombination protein RecT	-
CRN68_RS05375	786405..789005	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
CRN68_RS05380	789107..789382	-	276	WP_001344816.1	hypothetical protein	-
CRN68_RS05385	789457..789627	-	171	WP_001352098.1	conserved protein, Rac prophage	-
CRN68_RS05390	789627..789848	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	783620..830622	47002

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T86457 WP_001443846.1 NZ_CP023960:c785547-785398 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T86457 NZ_CP023960:c785547-785398 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT86457 NZ_CP023960:785176-785354 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin